The potential synergistic activity of chitosan-essential oils combination for fighting multidrug-resistant Salmonella Typhimurium and Staphylococcus aureus

Document Type : Original Article


1 Agric. Microbiology Dept., Fac. Agric. Moshtohor, Benha Univ., Egypt

2 Agric. Microbiology Dept., Fac. Agric., Ain Shams Univ., Egypt


Multidrug resistance among bacteria is now one of the most pressing issues in global public health. So, novel and more effective antibacterial materials are needed to address this challenge. The current study aims to use natural antibacterial agents and new strategies to prevent the growth of multidrug-resistant Salmonella Typhimuṛium and Staphylococcus aureus. Ten essential oils (EOs) and their mixture, chemical preservatives, chitosan, nano-chitosan, chitosan solution/film loaded with Eos were tested as antibacterial agents against pathogenic bacterial strains. Salmonella Typhimurium ATCC 25566 and Staphylococcus aureus ATCC 6538 were the most resistant to several antibiotics. Each essential oil of turmeric, cumin, pepper black, and marjoram, had no effect on S. Typhimurium while Staph. aureus was sensitive to them. However, clove, thyme, cinnamon, and garlic EO showed the maximum effect on S. Typhimurium and Staph. aureus. Their minimal inhibition concentration (MIC) was (350, 400, 350, and 500 μl 100-1) against S. Typhimurium and (250, 350, 250, and 400 μl 100-1 ml) against Staph. aureus, respectively. The mixture of clove and thyme recorded higher antibacterial activity values against S. Typhimurium and Staph. aureus compared to the mixture of other oils. Chitosan and nano-chitosan demonstrated potent antibacterial activity against S. Typhimurium and Staph aureus. Staph. auṛeus was more sensitive to nano chitosan. The mixture solution of chitosan, clove, and thyme was the most active combination against S. Typhimurium and Staph. aureus. Biodegradable chitosan film loaded with EOs was more effective antibacterial activity against S. Typhimurium and S. aureus than chitosan-free-essential oils films.


Main Subjects