Document Type : Original Article
Authors
1
Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Qalyoubeia 13736, Egypt
2
Soil and Water Research Department, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt
3
Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority
4
Soil and Water Research Department, Nuclear Research Center, Atomic Energy Authority Cairo, Egypt
5
3 Ahmed Elzomer Nacer City, Egypt
Abstract
Different samples used for Streptomyces spp. isolation. Obtained isolates examined for their zinc solubilization efficiency % (ZSE %), phosphate solubilization efficiency (PSE), siderophore production, indole acetic acid (IAA), cytokinin, and gibberellin production capacity. The most potent three isolates were genetically identified by 16S rRNA gene sequence analysis. The identified isolates were related to Streptomyces alfalfae strain XY25, Streptomyces litmocidini strain NRRL B-3635 and Streptomyces hawaiiensis strain ISP 5042. The spores of S. alfalfae, S. litmocidini, and S. hawaiiensis were exposed to increasable doses of 60Co Ɣ-rays (5, 10, 15, and 20 kGy), according to the climatic changes, the effect of irradiation formed a stress condition, so that examination the effect of irradiation on the most efficacious three strains' activities. Amongst the four radiation doses, e.g., 5 kGy, 10 kGy, 15 kGy and 20kGy, the 15 kGy Ɣ-rays show the most effective radiation dose, especially for the 1st or 2nd generation activities of S. alfalfae. This is a new study that uses high radiation dose treatments to enhance the activity of S. alfalfae, S. litmocidini and S. hawaiiensis. Because as far as our knowledge, this is the first report on their activity.
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