Document Type : Original Article
Authors
1
Soils Department, Faculty of Agriculture, Mansoura University, Egypt
2
Bioprocess Development Dept., Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, 21934 Alexandria, Egypt
3
Agricultural Chemistry Department, Faculty of Agriculture, Mansoura University
4
Soils Dept., Faculty of Agriculture, Damietta University, 34517 Damietta, Egypt
5
Plant Biotechnology Dept., Genetic Engineering and, Biotechnology Institute, Sadat City University, 32958 Sadat, Egypt
6
Agr. Microbiology Department, Fac. of Agriculture, Mansoura university, Mansoura, Egypt.
7
Soil and Water Dept., Kafrelsheikh Uni.
Abstract
The generation of new elements inside living cells, either by nuclear fusion or nuclear fission is questionable. Nuclear fusion theory can interpret the increase of an element or reduction of another one following the dissociation of the atomic nucleus of some elements and recoordination of new integrated nuclei, which surrounded by electrons to neutralize protons of nucleus. To proof our hypothesis, in vitro microcosm investigations were carried out using two microbial stains (Bacillus amyloliquefaciens MN592674B and Escherichia coli), which spiked with seven trace elements (Ti, V, Co, Ni, Se, Mo, and B) in a closed system. Total trace element concentrations were determined using Inductivity Coupled Plasma Optical Emission Spectrometry (ICP-OES) at the end of inoculation experiment. Some trace elements (e.g. Se, V and Ni) showed increments, others (e.g. Ti and B) showed reductions; however, some trace elements such as Ag, B and Cd vanished completely from the microbial media. The addition of V, Co, Ni, Se, Mo, and B to B. amyloliquefaciens culture medium enhanced Bismuth (Bi) biosynthesis. This finding was also supported by Energy Eispersive X-ray Spectroscopy (EDS) data, which showed substantial alterations in metals distribution in the outersphere surfaces as affected by the microbial strain and the spiked elements. In this regard, trace elements transformation differed greatly among bacterial strains. Findings from this investigation provide insights for understanding elemental transformations in the living cells.
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